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View full-text article in PMC

. 2009 Oct 12;9:362. doi: 10.1186/1471-2407-9-362

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Copyright ©2009 Tuomela et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Metastasis of PC-3/VEGF-C tumors to prostate-draining lymph nodes and lungs. The prostate draining lymph nodes and the lungs were cut through to detect metastasis. Altogether 8-10 lymph nodes and six lung sections/mouse were studied and number of metastases was counted. The relative metastatic area of lymph nodes was determined by histomorphometry but there were no statistically significant differences (data not shown). The occurrence of metastasis in lymph nodes was 21% (n = 6 mice out of 29 tumor-bearing mice) in the PC-3/VEGF-C group and 58% (n = 14 mice out of 24 tumor-bearing mice) in the PC-3/mock group and in lungs was 48% (n = 14 out of 29 tumor bearing mice) in the PC-3/VEGF-C group and 8% (n = 2 out of 24 tumor-bearing mice) in the PC-3/mock group.