Table 1.
List of phage displayed peptides and their corresponding MmmSC proteins identified after selection with the indicated antibody subpopulations from animal C11 and the Botswana outbreak
| Mmm SC protein match | Identified by serum from: | Accession numbera | Locus Tag | |
|---|---|---|---|---|
| Animal C11c | Botswana outbreakc | |||
| IgG panning | ||||
| Putative variable surface protein | Yes (3) | ND | CAE77433 | MSC_0818 |
| Cell division protein (FtsZ) | Yes (1) | ND | CAE77211 | MSC_0588 |
| ABC transporter, substrate-binding component (Abc) | Yes (1) | ND | CAE77420b | MSC_0804 |
| Prolipoprotein (Lpp) | Yes (1) | ND | CAE77256 | MSC_0635 |
| D-lactate dehydrogenase (Ldh) | Yes (2) | ND | CAE76687 | MSC_0034 |
| DNA gyrase subunit A (GyrA) | Yes (2) | ND | CAE76660 | MSC_0007 |
| DNA gyrase subunit B (GyrB) | Yes (1) | ND | CAE76659 | MSC_0006 |
| PTS system, glucose-specific, IIBC component (PtsG) | Yes (4) | ND | CAE77485b | MSC_0873 |
| IgG2 panning | ||||
| Transposase ISMmy1C | Yes (1) | No | CAE77424 | MSC_0808 |
| 30S ribosomal protein S5 (RpsE) | Yes (1) | No | CAE77346 | MSC_0728 |
| Putative glucose-specific IIABC component (PtsG) | Yes (1) | No | CAE76806 | MSC_0161 |
| Prolipoprotein (Lpp) | Yes (1) | No | CAE77256 | MSC_0635 |
| Prolipoprotein LppC (LppC) | Yes (1) | No | CAE76772 | MSC_0122 |
| Alanine-tRNA ligase (AlaS) | Yes (1) | No | CAE76823 | MSC_0178 |
| DNA gyrase subunit B (GyrB) | Yes (1) | Yes (1) | CAE76659 | MSC_0006 |
| Methionine adenosyltransferase (MetK) | Yes (1) | No | CAE77120 | MSC_0492 |
| Glyceraldehyde-3-phosphate dehydrogenase (GapN) | Yes (1) | No | CAE77137b | MSC_0509 |
| Glycerol-3-phosphate oxidase (GlpO) | Yes (1) | Yes (1) | CAE76900b | MSC_0259 |
| D-lactate dehydrogenase (Ldh) | Yes (1) | No | CAE76687 | MSC_0034 |
| P115-like protein with SMC_C motif | Yes (7) | No | CAE77104 | MSC_0476 |
| Ribose/Galactose ABC transporter, ATP-binding component (RbsA) | Yes (3) | No | CAE76663 | MSC_0010 |
| Prolipoprotein B (LppB) | Yes (2) | Yes (5) | CAE77147b | MSC_0519 |
| Glucose inhibited division protein A (GidA) | Yes (1) | No | CAE77646 | MSC_1042 |
| ABC transporter, substrate-binding component (Abc) | Yes (4) | Yes (1) | CAE77420b | MSC_0804 |
| Fructose-bisphosphate aldolase class II (FbaA2) | Yes (1) | No | CAE76786 | MSC_0139 |
| IgA panning | ||||
| Glyceraldehyde-3-phosphate dehydrogenase (NADP) (GapN) | Yes (1) | No | CAE77137b | MSC_0509 |
| Prolipoprotein B (LppB) | No | Yes (3) | CAE77147b | MSC_0519 |
| ABC transporter, substrate-binding component (Abc) | No | Yes (2) | CAE77420b | MSC_0804 |
| CTP synthase (CtrA) | Yes (1) | No | CAE76781 | MSC_0134 |
| Prolipoprotein LppC (LppC) | No | Yes (1) | CAE76772 | MSC_0122 |
| Thioredoxin reductase (NADPH) (TrxB) | Yes (1) | No | CAE77547 | MSC_0938 |
| Asparagine-tRNA ligase (AsnS) | Yes (1) | No | CAE76732 | MSC_0080 |
a EMBL/GenBank accession numbers for the protein sequences.
b Proteins that were expressed in this study (see Figure 3).
c Figures in parenthesis indicate numbers of peptides selected
ND - not determined