Fig. 3.

The interaction between the C protein/mutants and the L protein detected by co-immunoprecipitation assay. The HeLa cells, preinfected with the vaccinia virus vTF7-3, were transfected with C protein/mutants encoding plasmids and the L protein encoding plasmid. At 24 h post-transfection, the cells were lysed and incubated with the anti-flag M2 affinity gel overnight. The affinity gel was washed and subsequently boiled with SDS-PAGE loading buffer and was subjected to Western blot analyses using anti-flag (A) and anti-L (B) antibodies, respectively. Mock: both L protein and C protein are absent; L only: C protein is absent. The intensity of each band was quantified using ImageQuant 5.2 software and the L/C ratio was measured as indicated below the figure.