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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: Virology. 2009 Sep 11;394(1):47–56. doi: 10.1016/j.virol.2009.08.024

Figure 2. SIVNef binds SIVGagPol via its p6*PR peptide.

Figure 2

A. GST pull-downs. They were performed with GST or GST.SIVNef and [35S]-labeled Gag, the frameshifted, protease negative fsSIVGagPR- and SIVp6*PR- peptides. Lanes 1, 4 and 7 represent 10% input for fsSIVGagPR-, SIVp6*PR- and SIVGag, respectively (boxed). Inputs for GST and GST.Nef are related to panel B. B. Reverted mutant SIVNef proteins restore the binding to SIVGagPol. GST pull-downs were performed with GST alone or GST.NefM8 and reverted GST.SIVNef and the Myc epitope-tagged fsSIVGagPR- proteins. Proteins were separated by 10% SDS-PAGE followed by western blotting with anti-Myc antibodies. Lane 1 represents 10% of the input for fsSIVGagPR-.