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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: Virology. 2009 Sep 11;394(1):47–56. doi: 10.1016/j.virol.2009.08.024

Figure 6. WT SIVNef and reverted mutant SIVNefM8 proteins bind Alix in cells.

Figure 6

293T cells co-expressed the HA epitope-tagged Alix protein and V5 epitope-tagged WT SIVNef, NefA16 or reverted mutant Nef proteins. Cell lysates were immunopreciptated with anti-V5 antibodies, followed by western blotting with antibodies against HA (IP, WB). Lower panels present expression levels for the proteins used in these experiments (inputs)(WB).