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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: Virology. 2009 Sep 11;394(1):47–56. doi: 10.1016/j.virol.2009.08.024

Figure 7. GagΔp6.SIVNef restores the release of viruses from the LD-deficient HIVΔp6 provirus.

Figure 7

293T cells co-expressed the LD-deficient NLHXΔp6 (HIVΔp6) virus together with the WT Gag, mutant GagΔp6 or GagΔp6.SIVNef proteins. Protein content in cell (Lysate) and viral (Virus) lysates was analyzed by western blotting with antibodies against p24 (HIVCA). Supernatants of transfected cells were examined for viral infectivity in TZM-bl cells. Values are presented as numbers of blue foci/ml in the lower panel. Data are representative of three experiments performed in duplicate with variations depicted by error bars.