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. Author manuscript; available in PMC: 2010 Oct 30.
Published in final edited form as: Vaccine. 2009 Jun 24;27(46):6432–6434. doi: 10.1016/j.vaccine.2009.06.015

Figure 2.

Figure 2

HPV-16 L2 in C3 cells: (A) DNA was isolated from C3 tumor cells and the L2 gene was amplified using different primer pairs. Lane 1 negative control, Lane 2, 3, and 4, amplified L2 fragments of 509 bp, 802 and 885 bp, respectively, Lane 5 amplification of the L2 gene (1.4kb). (B) C3 cells were lysed and run on a PAGE gel, blotted, and probed with L2 antibody. Lane 1 L2 expressed in E. coli, Lanes 2–4 increasing amounts of 5, 10 and 15 μl of cell lysate was run.