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. Author manuscript; available in PMC: 2010 Nov 1.

Published in final edited form as: Microb Pathog. 2009 Aug 26;47(5):258–266. doi: 10.1016/j.micpath.2009.08.004

Time-course studies of the invasion and intracellular multiplication of L. monocytogenes in TIB73 cells after BNF treatment. TIB73 cells were pretreated with BNF (10 μM), 3′-MNF (1 μM), or DMSO for 5 h before being infected with 10⁵ log-phase L. monocytogenes for 2 h (Ingestion). Cells were then washed extensively to remove extracellular Listeriae and incubated in media with 5 μg/mL gentamycin for 2 to 24 h. A. CFU of L. monocytogenes recovered from infected TIB73 cells: Infected cells were lysed in sterile H₂O to determine the CFU. Symbols indicate: BNF (10 μM) (), 3′-MNF (1 μM) (), or DMSO (). Data represent the mean±SEM Log₁₀CFU/mL of six independent experiments. *: p<0.05, as compared to the control group. B. Fold change in CYP1A1 mRNA after being normalized to the control (GAPDH). Identically-treated TIB73 cells were used to isolate RNA for real-time RT-PCR analyses of CYP1A1. Data represent mean±SEM fold change of six independent experiments that were performed. Symbols indicate BNF (10 μM) (), 3′-MNF (1 μM) (), or DMSO ().

Inline graphic — Time-course studies of the invasion and intracellular multiplication of L. monocytogenes in TIB73 cells after BNF treatment. TIB73 cells were pretreated with BNF (10 μM), 3′-MNF (1 μM), or DMSO for 5 h before being infected with 10⁵ log-phase L. monocytogenes for 2 h (Ingestion). Cells were then washed extensively to remove extracellular Listeriae and incubated in media with 5 μg/mL gentamycin for 2 to 24 h. A. CFU of L. monocytogenes recovered from infected TIB73 cells: Infected cells were lysed in sterile H₂O to determine the CFU. Symbols indicate: BNF (10 μM) (), 3′-MNF (1 μM) (), or DMSO (). Data represent the mean±SEM Log₁₀CFU/mL of six independent experiments. *: p<0.05, as compared to the control group. B. Fold change in CYP1A1 mRNA after being normalized to the control (GAPDH). Identically-treated TIB73 cells were used to isolate RNA for real-time RT-PCR analyses of CYP1A1. Data represent mean±SEM fold change of six independent experiments that were performed. Symbols indicate BNF (10 μM) (), 3′-MNF (1 μM) (), or DMSO ().