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. Author manuscript; available in PMC: 2010 Nov 1.

Published in final edited form as: Microb Pathog. 2009 Aug 26;47(5):258–266. doi: 10.1016/j.micpath.2009.08.004

BNF dose-dependently inhibits the invasion and intracellular growth of L. monocytogenes in TIB73 cells. TIB73 cells were treated with 1 (), 5 (), or 10 μM () of BNF for 5 h prior to infection with 10⁵ log-phase L. monocytogenes for 2 h (Ingestion). Cells were then washed extensively to remove extracellular Listeriae and incubated in media with 5 μg/mL gentamycin for an additional 8 h. DMSO was included as a vehicle control (). A. Infected cells were lysed in sterile water to quantify the number of viable L. monocytogenes. Data represent the mean±SEM Log₁₀CFU/mL of four independent experiments that were performed. *: p<0.05 in comparison to the vehicle control. B. Parallel sets of TIB73 cells were used to isolate total RNA for real-time RT-PCR analyses of CYP1A1. CYP1A1 mRNA expression was presented as the mean±SEM fold change, relative to control samples, after normalization to that of GAPDH in the same samples.

Inline graphic — BNF dose-dependently inhibits the invasion and intracellular growth of L. monocytogenes in TIB73 cells. TIB73 cells were treated with 1 (), 5 (), or 10 μM () of BNF for 5 h prior to infection with 10⁵ log-phase L. monocytogenes for 2 h (Ingestion). Cells were then washed extensively to remove extracellular Listeriae and incubated in media with 5 μg/mL gentamycin for an additional 8 h. DMSO was included as a vehicle control (). A. Infected cells were lysed in sterile water to quantify the number of viable L. monocytogenes. Data represent the mean±SEM Log₁₀CFU/mL of four independent experiments that were performed. *: p<0.05 in comparison to the vehicle control. B. Parallel sets of TIB73 cells were used to isolate total RNA for real-time RT-PCR analyses of CYP1A1. CYP1A1 mRNA expression was presented as the mean±SEM fold change, relative to control samples, after normalization to that of GAPDH in the same samples.