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. Author manuscript; available in PMC: 2010 Nov 1.

Published in final edited form as: Microb Pathog. 2009 Aug 26;47(5):258–266. doi: 10.1016/j.micpath.2009.08.004

Knocking down AhR with siRNAs did not abolish BNF inhibition. Low-confluence TIB73 cells (∼20%) were transfected with a set of four ON-TARGETplus siRNAs against mouse AhR, or non-specific scrambled siRNAs, using Lipofectamine 2000. After 72 h, siRNA-treated TIB73 cells were used to isolate total protein (A), or were subjected to BNF treatment (B). A. AhR protein expression in TIB73 cells was decreased greater than 90% following the treatment with a mixture of 4 ON-TARGETplus siRNA duplexes (numbered 1-4) (Western Blot). B. Scrambled siRNA-transfected TIB73 cells treated with DMSO () or BNF (10 μM) (), or ON-TARGET siRNA-transfected TIB73 cells treated with DMSO () or BNF (10 μM) (), were infected with L. monocytogenes, as described in the methods. The data depict the mean±SEM Log₁₀CFU/mL of three independent experiments that were performed. *: p<0.05, as compared to the corresponding DMSO controls.

Inline graphic — Knocking down AhR with siRNAs did not abolish BNF inhibition. Low-confluence TIB73 cells (∼20%) were transfected with a set of four ON-TARGETplus siRNAs against mouse AhR, or non-specific scrambled siRNAs, using Lipofectamine 2000. After 72 h, siRNA-treated TIB73 cells were used to isolate total protein (A), or were subjected to BNF treatment (B). A. AhR protein expression in TIB73 cells was decreased greater than 90% following the treatment with a mixture of 4 ON-TARGETplus siRNA duplexes (numbered 1-4) (Western Blot). B. Scrambled siRNA-transfected TIB73 cells treated with DMSO () or BNF (10 μM) (), or ON-TARGET siRNA-transfected TIB73 cells treated with DMSO () or BNF (10 μM) (), were infected with L. monocytogenes, as described in the methods. The data depict the mean±SEM Log₁₀CFU/mL of three independent experiments that were performed. *: p<0.05, as compared to the corresponding DMSO controls.