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. Author manuscript; available in PMC: 2010 Nov 1.

Published in final edited form as: Microb Pathog. 2009 Aug 26;47(5):258–266. doi: 10.1016/j.micpath.2009.08.004

BNF inhibits the ingestion and intracellular multiplication of L. monocytogenes in AhR-deficient and ARNT-dysfunctional Hepa cells. Hepa-1 (wild type) and AhR-deficient and ARNT-dysfunctional Hepa cells were treated with DMSO or BNF (10 μM) for 5 h before being infected with 10⁵ log-phase L. monocytogenes for 2 h (Ingestion). Cells were then washed extensively to remove extracellular Listeriae and incubated in media with 5 μg/mL gentamycin for 8 h. Cells were then lysed in sterile H₂O to determine the CFU of L. monocytogenes. Data illustrate the mean±SEM Log₁₀CFU/mL of three separate experiments that were performed. *: p<0.05, as compared to the corresponding controls. A. BNF () inhibited the ingestion and intracellular growth of L. monocytogenes in the WT Hepa-1 cells at all the times tested as compared to the DMSO control (). B. The inhibitory effects of BNF on L. monocytogenes persist as seen in AhR-deficient and ARNT-dysfunctional Hepa cells. Used symbols indicate: : Ingestion, DMSO control; : Ingestion, BNF (10 μM); : 8 h, DMSO control; : 8 h, BNF (10 μM).

Inline graphic — BNF inhibits the ingestion and intracellular multiplication of L. monocytogenes in AhR-deficient and ARNT-dysfunctional Hepa cells. Hepa-1 (wild type) and AhR-deficient and ARNT-dysfunctional Hepa cells were treated with DMSO or BNF (10 μM) for 5 h before being infected with 10⁵ log-phase L. monocytogenes for 2 h (Ingestion). Cells were then washed extensively to remove extracellular Listeriae and incubated in media with 5 μg/mL gentamycin for 8 h. Cells were then lysed in sterile H₂O to determine the CFU of L. monocytogenes. Data illustrate the mean±SEM Log₁₀CFU/mL of three separate experiments that were performed. *: p<0.05, as compared to the corresponding controls. A. BNF () inhibited the ingestion and intracellular growth of L. monocytogenes in the WT Hepa-1 cells at all the times tested as compared to the DMSO control (). B. The inhibitory effects of BNF on L. monocytogenes persist as seen in AhR-deficient and ARNT-dysfunctional Hepa cells. Used symbols indicate: : Ingestion, DMSO control; : Ingestion, BNF (10 μM); : 8 h, DMSO control; : 8 h, BNF (10 μM).