Fig. 1.

Restriction fragment length polymorphism (RFLP) analysis of the product of a polymerase chain reaction (PCR) targeting VDR. Pattern of fragments for the three possible genotypes after Taq I digestion of the PCR product, a 740-bp amplified region of the VDR gene. The 245-bp fragment is constant in all genotypes, being created by cleavage at a nonpolymorphic Taq I site within the range of amplification. Lane 1TT genotype (2 fragments of 495 and 245 bp), Lane 2tt genotype (3 fragments of 290, 245, and 205 bp), Lane 3Tt genotype (4 fragments of 495, 290, 245, and 205 bp)