Figure 2.
Multiple amino acid sequence alignment of several Class I flavin reductases. Abbreviations, GenBank accession numbers, and % sequence identity/similarity to SgcE6 are as follows: SgcE6 (AAL06698); PheA2, phenol 2-hydroxylase component B from B. thermoglucosidasius A7 (AAF66547, 35/56); EC-HpaC, 4-hydroxyphenylacetate hydroxylase component C from E. coli (CAA82322, 26/46); SV-VlmR, flavin reductase involved in valanimycin biosynthesis in Streptomyces viridifaciens (AAC45645, 28/46); SC-ActVB, flavin reductase component B involved in actinorhodin biosynthesis in Streptomyces coelicolor (CAA45048, 40/51); RebF, flavin reductase involved in rebeccamycin biosynthesis (CAC93720, 34/48); KtzS, flavin reductase involved in kutzneride biosynthesis (ABV56599, 33/46); BC-TftC, flavin reductase component of chlorophenol 4-hydroxylase from Burkholderia cepacia (AAC23547, 27/43); EC-Fre, E. coli flavin reductase (AAA23806, 11/23); VF-FRaseI, flavin reductase from Vibrio fischeri (BAA04595, 11/22). Shaded residues are conserved among the HpaC-like subfamily, and the boxed regions denote subfamily consensus motifs.