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. Author manuscript; available in PMC: 2009 Oct 27.
Published in final edited form as: J Am Soc Mass Spectrom. 2008 Jul 1;19(10):1459–1466. doi: 10.1016/j.jasms.2008.06.021

Figure 3.

Figure 3

RP-HPLC/ESI-MS analysis of sulfated and nonsulfated CCR8 12–20 standard peptides. (a) Full scan analysis of the IS, monosulfated product B, and disulfated product C showing their respective singly-charged (insert) and doubly-charged species. (b) Total ion chromatogram (TIC) showing the separation and order of elution of the 4 CCR8 peptides. (c) Mass spectrum in the selected ion monitoring (SIM) mode showing the most abundant signals for the doubly-charged ions of the 4 CCR8 peptides. A = nonsulfated CCR8, B = sY15CCR8, C = sY15sY17CCR8