Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In vivo labeling strategy. Cells are grown in the presence of azido-pantetheine (1) or fluorescent pantetheine (2). After uptake, the native CoA biosynthetic enzymes convert the pantetheine analogues to CoA analogues which then are appended to endogneous CPs via the native PPTase enzyme. After cell lysis azido-modified CPs can be detected by reaction with alkyne probes (3–5).
