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Detection of in vivo modified carrier proteins. (A) Cultures were grown with or without compound 1 and reacted with RRX-alkyne 4 after lysis. Distinct CP labeling is seen in E. coli (1), B subtilis 168 (2), B. subtilis 6051 (3), and S. oneidensis (4) as compared to negative controls (B) Labeling of E. coli ACP by 1 with visualization by DMAC-alkyne 3 was effective in both LB (lane 1) and M9 minimal media (lane 2) as compared to negative controls (lanes 2,4).
