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. Author manuscript; available in PMC: 2009 Oct 27.
Published in final edited form as: Chembiochem. 2009 Apr 17;10(6):1091–1100. doi: 10.1002/cbic.200800838

Figure 4.

Figure 4

Affinity purification of ACP. Lysate from cultures of S. oneidensis MR1 grown with compound 1 and then treated with biotin-alkyne 5 were incubated with streptavidin agarose allowing for purification of modified CP (crude lysate lane 1, desaltting column flow lane 2, recovered ACP band—between white arrows—10 kDa lane 3). Cultures grown with compound 1 but not reacted with biotin-alkyne 5 (lanes 4,5,6) or grown without compound 1 (lanes 7,8,9) showed no protein enrichment after incubation with streptavidin agarose. The strong band below ACP is a contaminate from the resin and was seen under all elution condtions.