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. Author manuscript; available in PMC: 2009 Oct 27.
Published in final edited form as: Mol Imaging. 2008 Jul–Aug;7(4):175–186.

Figure 4. Microcalcification formation in untransduced, contralateral tumors.

Figure 4

Figure 4

Figure 4

a. MicroCT imaging of bilateral tumors. Rats inoculated with bilateral tumors were scanned using microCT at 4 weeks. U = contralateral tumor whose R3230 cells were untransduced prior to inoculation. B = ipsilateral tumor whose R3230 cells were transduced with AdGFP/BMP-2 prior to inoculation. Percentage of microcalcifications per tumor (mean ± SEM) is shown for N = 4 animals.

b. RT-PCR analysis of bilateral tumors. Tumors excised 4 weeks after inoculation were subjected to RT-PCR analysis on a 2.5% agarose gel. Lane 1 = hBMP-2 (377 bp); Lane 2 = GFP (312 bp); Lane 3 = partial of BMP-2 sequence and SV40 polyA sequence from adenovirus (280 bp); Lane 4 = rat BMP-2 (374 bp); rat GAPDH (270 bp).

c. Expression of GFP in ipsilateral and contralateral tumors. Cohorts of N = 8 Fischer 344 rats with bilateral R3230 tumors as described in Figure 4a were sacrificed weekly. Tumors were bisected and imaged using white light (left) or GFP fluorescence (right). U = contralateral tumor whose R3230 cells were untransduced prior to inoculation. B = ipsilateral tumor whose R3230 cells were transduced with AdGFP/BMP-2 prior to inoculation. GFP fluorescence images have identical exposure times and normalizations.