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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Virology. 2008 Dec 10;385(1):183–191. doi: 10.1016/j.virol.2008.11.018

Fig. 2. Expression and ubiquitination of LMP2A signalosome.

Fig. 2

(A) BJAB and CHO-K1 cells were transfected with 10 or 1 μg of LMP2A, Lyn, Syk-Myc and c-Cbl-Flag, respectively. Cell lysates were immunoblotted with anti-LMP2A, Lyn, Syk or c-Cbl antibody to detect both endogenous and transfected expression. (B) BJAB cells were transfected with 10 μg of LMP2A, Ub-HA and c-Cbl-Flag, respectively. LMP2A immunoprecipitates were immunoblotted with anti-HA antibody to detect ubiquitin-conjugated LMP2A. Cell lysates were immunoblotted with anti-c-Cbl antibody. The data is the representative of four comparable experiments.