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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Virology. 2008 Dec 10;385(1):183–191. doi: 10.1016/j.virol.2008.11.018

Fig. 5. LMP2A-dependent Syk degradation by c-Cbl.

Fig. 5

(A) CHO-K1 cells were transfected with indicated amount of LMP2A-HA, 1 μg of Syk-Myc, and 1 μg of c-Cbl-Flag. Cell lysates were immunoblotted for LMP2A, Syk and c-Cbl. (B) CHO-K1 cells were transfected with 1 μg of wild type or ITAM-mutant LMP2A-HA, Syk-Myc, c-Cbl-Flag and Ub-His. Anti-Syk (Myc) immunoprecipitates were immunoblotted with anti-His antibody to detect ubiquitin-conjugated Syk. The data is the representative of three comparable experiments.