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. Author manuscript; available in PMC: 2010 Mar 6.
Published in final edited form as: J Mol Biol. 2009 Mar 6;386(4):1123–1137. doi: 10.1016/j.jmb.2009.01.018

Figure 6.

Figure 6

Figure 6

Figure 6

Figure 6

Figure 6

Figure 6

The effect of zinc binding on HPV E6 structure. (A), (B), and (C) show the CD spectra of GBF-E6, GBF-E6N, and GBF-E6C and (D), (E), and (F) show the fluorescence spectra of GBF(W43′F)-E6, GBF-E6N, and GBF(W43′F)-E6C. GBF(W43′F)-E6 was 5 μM and GBF-E6N and GBF(W43′F)-E6C were 10 μM. The buffers used were 50 mM phosphate, pH 6.50 (black), 50 mM phosphate, pH 6.50 with 8 mM Zn-EGTA (red), 50 mM phosphate, pH 6.50 with 8 mM EGTA (green), 50 mM phosphate, pH 6.50 with 8 mM EDTA (blue).