Table 2. HTB49 CM spot identities.
| Name | Accession no. | No of spots | No. of matched peaks | No. of unmatched peaks | % Coverage | MASCOT Score | MSMS | Status |
|---|---|---|---|---|---|---|---|---|
| IGFBP-rP1 | Q16270 | 1 | 14 | 66 | 45.0 | 120 | SiP | |
| Reticulocalbin 1 precursor | Q15293 | 1 | 9 | 42 | 42.0 | 80 | SiP† | |
| SPARC | P09486 | 1 | 9 | 42 | 32.0 | 70 | SiP† | |
| Vimentin | P08670 | 1 | 35 | 67 | 71.0 | 291 | SeP | |
| PAI-1 | P05121 | 4 | 18 | 30 | 49.9 | 157 | 2 | SiP |
| Transferrin | P02787 | 8 | 13 | 27 | 30.0 | 105 | ||
| Cathepsin D | P07339 | 5 | 9 | 28 | 32.1 | 83 | SiP† | |
| Nucleobindin 1 | Q02818 | 1 | 22 | 56 | 54.0 | 172 | SiP | |
| BIGH3 | Q15582 | 5 | 20 | 33 | 41.5 | 166 | 2 | SiP |
| Complement C3 | P01024 | 1 | 20 | 32 | 23.8 | 138 | SiP |
From HTB49 CM, 27 spots (10 proteins) upregulated vs whole cell lysate were identified. Where a protein was found in more than one spot, a representative example of scores is shown. Confirmation of peptide mass finger printing (PMF) by MS/MS was undertaken for some spots and this is indicated by the number of significant peptides found. Proteins predicted to contain a signal sequence using SignalP 3.0 are denoted SiP and those predicted to undergo non-classical secretion using SecretomeP are denoted SeP. In the Status column, proteins marked † indicate those proteins upregulated in comparison to normal conditioned media (CM) (further proteins identified are listed in Supplementary Table 1).