Figure 5.

Determination of the effects of S100A6 knockdown on cell proliferation. (A) [³H]-thymidine incorporation was performed 72 h after transfection with 10 nm S100A6-targeting and control siRNAs. Histogram showing [³H]-thymidine incorporation in S100A6 depleted compared with control-siRNAs-treated Panc-1 cell line. Error bars represent standard error for five experiments performed in triplicate. (B) Panc-1 cells were plated 72 h after transfection with S100A6-targeting and control siRNAs and 18 h later MTT assays were performed. Results were expressed as MTT reading after siRNA treatment/MTT reading after RISC-free treatment. Error bars represent standard error for five experiments performed in triplicate.