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. 2009 Sep 29;101(7):1100–1106. doi: 10.1038/sj.bjc.6605296

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Copyright © 2009 Cancer Research UK

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Inhibitory effect of Ki23057 on VEGFR-3 phosphorylation. (A) The inhibitory effect of Ki23057 on the phosphorylation of VEGFR-3 in gastric tumour specimens. VEGFR-3 phosphorylation was suppressed in the tumours of the Ki23057-treated mice (mouse 2 and mouse 4), in comparison with that in the vehicle mice (mouse 1 and mouse 3). (B) The effects of Ki23057 on VEGFR-3 phosphorylation levels on lymphatic endothelial cells. To assess the phosphorylation of VEGFR-3 in tumour tissue, the anti-Flt-4 antibody reacts with mouse VEGFR-3 and phosphotyrosine was examined. Tumours treated by Ki23057 showed an apparent attenuation of phosphorylation (arrows) on the lymphatic endothelial cells of VEGFR-3 staining (arrowheads), in comparison with that in vehicle mice.