A. Shown is the time-course of the effect of 50 mM EtOH on mIPSC frequency. The average mIPSC frequency in 10 s bins was obtained. These data were subsequently normalized with respect to the average mIPSC frequency obtained during the first 10 s of recording (n = 10). B. EtOH acutely increased mIPSC frequency in a concentration-dependent manner (n = 4, 9 and 7 for 25, 50 and 60 mM, respectively). The effect of EtOH was calculated with respect to the average of baseline and washout responses (represented by the dotted line). **, p<0.002; ***, p<0.001 by one sample t-test vs. 100%