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. Author manuscript; available in PMC: 2009 Oct 27.
Published in final edited form as: Cancer Prev Res (Phila). 2008 Oct;1(5):316–328. doi: 10.1158/1940-6207.CAPR-07-0002

Figure 2. IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner.

Figure 2

A, A549 cells were incubated with control medium or with medium containing 2.5 ng/ml of IL-1β for the indicated times. Total RNAs were collected from the cells and expression of the indicated genes was measured using qPCR. GAPDH was used as an internal control for normalizing the RNA loading. B, A549 cells were incubated with IL-1β at the indicated concentrations for 8 h. Total RNAs were collected from the cells for qPCR. C, Culture media were collected from A549 cells with treatment as in (B) and the concentrations of CXCL5 and CXCL8 were measured with ELISA Data were expressed as the mean ± SE. *, p<0.05; **, p<0.01; ***, p<0.001 compared with untreated control.