A, A549 cells were incubated with control medium or with medium containing 2.5 ng/ml of IL-1β for the indicated times. Total RNAs were collected from the cells and expression of the indicated genes was measured using qPCR. GAPDH was used as an internal control for normalizing the RNA loading. B, A549 cells were incubated with IL-1β at the indicated concentrations for 8 h. Total RNAs were collected from the cells for qPCR. C, Culture media were collected from A549 cells with treatment as in (B) and the concentrations of CXCL5 and CXCL8 were measured with ELISA Data were expressed as the mean ± SE. *, p<0.05; **, p<0.01; ***, p<0.001 compared with untreated control.