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. Author manuscript; available in PMC: 2009 Oct 27.
Published in final edited form as: Cancer Prev Res (Phila). 2008 Oct;1(5):316–328. doi: 10.1158/1940-6207.CAPR-07-0002

Figure 6. KG-501 suppresses NSCLC/IL-1β CM-induced migration of HUVECs by regulating IL-1β-induced CXC chemokines gene expression in NSCLC cells.

Figure 6

A, HUVECs migration was induced with CM from A549 cells treated with control medium (a) or medium containing 2.5 ng/ml of IL-1β (b) or 10 µM of KG-501 (c) or 2.5 ng/mL of IL-1β plus 2.5 µM of KG-501 (d) or 2.5 ng/mL of IL-1β plus 5 µM of KG-501 (e) or 2.5 ng/mL of IL-1β plus 10 µM of KG-501 (f) in serum-free medium for 24h. B, Quantification of image analysis for the HUVECs migration assay. C, Total RNAs were isolated from cells with the treatment described in (A), and real-time PCR was performed to measure the expression level of the indicated genes. D, The concentration of CXCL5 and CXCL8 protein in the CM was measured with ELISA. Results were normalized according to the cell number. Data were expressed as the mean ± SE. **, p<0.01; ***, p<0.001.