FIG. 2.

Gene profiles of macrophages positive or negative for CD11c and CD206 in epididymal fat tissue. Cells in the SVF of epididymal fat tissue from mice fed a HFD for 12 weeks were analyzed using flow cytometry. F4/80-positive cells were further analyzed with anti-CD11c and anti-CD206 antibodies. A: Representative flow cytometry results. Blue dots show isotype control. Total RNA was isolated from F4/80-positive/CD11c-positive/CD206-negative cells or F4/80-positive/CD11c-negative/CD206-positive cells. B: Real-time RT-PCR for the indicated genes was conducted as described in the research design and methods section. Data were normalized according to the 18S mRNA level. The results are shown as the means ± SE for four to seven animals per group. *P < 0.05; **P < 0.01. The correlation between CD206 expression and MglI expression was examined. C: Representative results.