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Effect of HFD and pioglitazone on gene expressions of adipocyte fraction in epididymal fat tissue. Total RNA from the adipocyte fraction was isolated, and real-time RT-PCR for the indicated genes was conducted as described in the research design and methods section. Data were normalized according to the 18S mRNA level. The results are shown as the means ± SE for six animals per group. *P < 0.05.
