Table 3.
Genotype at HO | ||||
---|---|---|---|---|
Origin of isolates | Microsatellite profiles | HO/HO | HO/ho | ho/ho |
Clinical | Polyallelic (diploid) | 2 | 1 | 4 |
Monoallelic | 1 | 0 | 0 | |
Nonclinical | Polyallelic (diploid) | 4 | 0 | 1 |
Monoallelic | 23 | 0 | 0 |
Genotypes at the HO locus of 36 clinical and nonclinical Saccharomyces cerevisiae isolates with either exclusively monoallelic profiles at 12 examined microsatellite loci or polyallelic profiles suggestive of a diploid state of the isolates; the genotype at the HO locus was determined by analysing the sporulation proficiency of the isolates' segregants; sporulation proficient segregants were considered HO (homothallic) and sporulation deficient segregants were considered ho (heterothallic); isolates that exhibited 2:2 segregation of HO:ho were considered heterozygous at HO, and sporulation deficient segregants were tested for heterothallism and haploidy by mating type tests.