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. 2009 Sep 16;29(37):11511–11522. doi: 10.1523/JNEUROSCI.1514-09.2009

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Copyright © 2009 Society for Neuroscience 0270-6474/09/2911511-12$15.00/0

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Figure 8. — Increased spread of neutrophils during EAE with ablation of proliferating reactive astrocytes. A, B, Detail, merged two- or three-color fluorescence images of spinal cord white matter stained for the neutrophils marker, Ag4/7 (red), GFAP (green), and (DAPI blue) in EAE+TK/PBS (A1, A2) and EAE+TK/GCV (B1, B2). Note that in EAE+TK/PBS, a few positive neutrophils are present but confined to a perivascular cluster of infiltrating inflammatory cells (pvc) surrounded by tightly packed GFAP-positive astrocytes (AS) (A1, A2), whereas in EAE+TK/GCV, Ag4/7-positive neutrophils (red) are increased in number and together with other inflammatory cells (blue) spread widely in the white matter (wm) parenchyma in regions depleted of astrocytes (B1, B2). Scale bar, 35 μm.