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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: PET Clin. 2009 Jan 1;4(1):1–15. doi: 10.1016/j.cpet.2009.04.012

Figure 2.

Figure 2

Flow cytometry of a mouse 66 mammary adenocarcinoma labeled with BrdU (100 mg/kg) every 8 h over a 48 h period, i.e. ~2 tumor cell cycle times. BrdU+ cells are proliferating (P) cells. Because the BrdU labeling occurred over 2 cell cycle times, the cycling P cells in all phases of the cell cycle are labeled. Cells that are BrdU− are quiescent noncycling cells. The fluorescent probe, 7-AA distinguishes 66 cells that have a 2C DNA content (G1/G0 cells) from cells that have a 4C DNA content (G2/M). Cells in S phase have an intermediate DNA content and usually are a small fraction of the total cells in a solid tumor.