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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Mol Cancer Res. 2008 Nov;6(11):1766–1774. doi: 10.1158/1541-7786.MCR-08-0169

Figure 2. Dasatinib Blocks Invasion of Human Melanoma Cells.

Figure 2

A2058 (panel A) and 1205-Lu cells (panel B) were seeded in 0.2% serum-containing medium into the upper part of the matrigel invasion chambers. The lower chambers were filled with 100% conditioned medium containing 10% serum to promote invasion through the matrigel. Cells were treated immediately with DMSO vehicle control or increasing concentrations dasatinib as indicated. Twenty-four hours later, non-invading cells were removed and the invaded cells were fixed and stained. The experiment was repeated 2 more times, with similar results. Cells in at least 3 different areas of the membrane were counted (panel C) and the data represent the average and standard deviation of 3 independent experiments. To measure the effect of dasatinib on MMP-9 protein expression, A2058 cells were treated for 96 h with increasing concentration of dasatinib or DMSO control and supernatants were analyzed for total MMP-9 protein concentrations (panel D). In all cases, when treated samples are compared with corresponding controls, P < 0.05.