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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Mol Cancer Res. 2008 Nov;6(11):1766–1774. doi: 10.1158/1541-7786.MCR-08-0169

Figure 3. Dasatinib Does Not Inhibit Proliferation and Viability of Melanoma Cells.

Figure 3

Human melanoma cells (1000/well) were seeded in 96-well plates and exposed to increasing concentrations of dasatinib or DMSO vehicle control for 72 h (viability assay) or 96 h (proliferation assay). To determine viable cells (panel A), a tetrazolium compound was added to each well and the absorbance of the resulting formazan product was measured at 490 nm as per the supplier’s instructions (Promega). To determine total cell number (panel B), cells were first lysed and total lactate dehydrogenase enzyme concentration was then measured as per the supplier’s instructions (Promega). Percent of viable cells and percent of total cells (cell number) was normalized to DMSO-treated cells. Each bar represents the average and standard deviation of 4 individual measurements at each indicated concentration of dasatinib.