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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Mol Cancer Res. 2008 Nov;6(11):1766–1774. doi: 10.1158/1541-7786.MCR-08-0169

Figure 4. Dose- and Time-Dependent Effects of Dasatinib on Melanoma Cell Signaling.

Figure 4

To determine dose-dependent effects of dasatinib on signaling in human melanoma cells, A2058 cells were treated for 3 h with DMSO vehicle control or increasing concentrations of dasatinib as indicated (panel A). Three-hundred nanomolar dasatinib was added to A2058 cells to determine time-dependent effects on cell signaling from 0.5 to 180 min as indicated (panel B). The levels of activated (phospho) and total Src, FAK and p130CAS proteins were measured by Western blot analysis. Phospho-Src antibody detects SFKs phosphorylated at the equivalent of c-Src Tyr419, phospho-FAK antibody detects phosphorylation of Tyr576/577 in FAK, and phospho-CAS antibody detects phosphorylation of Tyr410 in p130CAS.