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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2009 Jan 27;8(2):458–468. doi: 10.1158/1535-7163.MCT-08-0885

Figure 1.

Figure 1

Ectopic expression of WIF1 in human urinary bladder cancer T24 and TSU-PR1 cells leads to a decrease of anchorage-dependent and anchorage-independent growth. A, WIF1 expression in cell lysates and conditioned medium of the representative stable transfectants was determined by Western blottingwith anti-human IgG. B, cell proliferation assay in bladder cancer T24 and TSU-PR1cells expressingpRK5 vector control or WIF1 at 1, 3, and 5 d after cell seeding. C, qualitative (inset) and quantitative analyses of soft agar colony formation in TSU-PR1 cells expressing pRK5 vector control or WIF1. Columns, mean of four independent wells at optimum time of 21 d after the start of cell seeding; bars, SE.