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. Author manuscript; available in PMC: 2009 Oct 27.

Published in final edited form as: Immunity. 2007 Aug 16;27(2):228–239. doi: 10.1016/j.immuni.2007.06.009

Nuclear Translocation of IRF-7 and IRF-3 in IRF-8^−/− DCs.

A: IRF-8^+/+ and ^−/− DCs were transduced with a vector for IRF-7-GFP and stimulated without (control) or with NDV for 1 h. Cells were fixed and stained with anti-GFP antibody and counterstained with Hoechst 33342 for DNA.

B: IRF-8^+/+ and IRF-8^−/− DCs were infected with NDV for 1 h as above and nuclear and cytoplasmic fractions were tested for indicated proteins by immunoblot analysis. c-cbl and YY1 were used to monitor the quality of fractionation.

C: IRF-8^+/+ and ^−/− DCs were stimulated with or without NDV and immunostained with antibody for endogenous IRF-3 as in A.

D: IRF-8^+/+ DCs stimulated with NDV for 7 h and whole cell extracts were tested for IRF-8 by immunoblot.