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. Author manuscript; available in PMC: 2009 Dec 12.
Published in final edited form as: Cell. 2008 Dec 12;135(6):1098–1107. doi: 10.1016/j.cell.2008.10.021

Figure 4. Droplets from Khc27/+ embryos have reduced Kinesin-1 levels and display lower stall forces.

Figure 4

(A) Khc levels on embryonic lipid droplets are reduced in Khc heterozygotes (Khc27/+) compared to the wild type (WT). Equal amounts of total protein from purified lipid droplets were analyzed for Khc by Western blotting. This trend was reproduced in independent preparations; in some of them equal loading was additionally verified by probing for LSD2 (data not shown).

(B, C) Average stall forces in Khc27/+ heterozygotes are reduced compared to the wild type, both for plus-end (B) and minus-end (C) moving droplets.

(D, F) Stall-force distribution in Khc27/+ heterozygotes for plus-end (D) and minus-end (E) moving droplets. Both histograms show a single prominent peak whose position coincides with the 2.6± 0.5 pN peak in Fig. 3C, within the uncertainty estimated as half the bin size. (plus-end: 1.9 ± 0.5 pN, minus-end 2.1 ± 0.5 pN). A diminished peak at 5.2 pN is expected given the reduced number of motors on the lipid droplets in heterozygous embryos compared to wild type.