Figure 3. Higher numbers of apoptotic keratinocytes are induced by UVB rradiation and epicutaneous ovalbumin sensitization in gal3−/− mice.
(a and b) Skin sections from gal3+/+ and gal3−/− mice were placed on gauze soaked in RPMI medium and irradiated with UVB ex vivo at the indicated doses. Twenty-four h after irradiation, samples were processed for TUNEL reaction. (c) Gal3+/+ and gal3−/− mice were treated with three one-week periods of epicutaneous application of ovalbumin or PBS, each two weeks apart. Skin sections were obtained from the treated areas 24 h after the third sensitization period and processed for TUNEL reaction. Apoptotic cells were counted per cm in the skin sections. Bars represent mean ± SD. *P<0.05.