Skip to main content
. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: Cell Host Microbe. 2009 Jan 22;5(1):47–58. doi: 10.1016/j.chom.2008.11.009

Figure 2.

Figure 2

Occludin and E-cadherin are targets for calpain cleavage. (A) Distribution of occludin (red) and E-cadherin (green) is altered following 6 h incubation with media, heat killed PAO1 or P3C. Cells were stained with DAPI (blue). Data is one representative of at least three separate experiments. (B) In the presence of 20 mM CaCl2, 1 μg of exogenous human calpain 1 cleaved occludin and E-cadherin but not JAM-1 or claudin-1 immunoprecipitated from 1HAEo- cell lysates. The exogenous calpain 1 in these reactions is autolysed upon activation in the presence of Ca2+. * Shorter exposure time on the three right bands compared to the three left bands. Data is one representative of at least three separate experiments.