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. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: Cell Host Microbe. 2009 Jan 22;5(1):47–58. doi: 10.1016/j.chom.2008.11.009

Figure 6.

Figure 6

PMN transmigration across airway epithelial monolayer is facilitated by calpain. (A) The number of PMNs that have migrated into the apical compartment of 16HBE monolayers stimulated with heat killed PAO1 or P3C in the presence or absence of calpeptin. (B) The migration of 106 calcein-AM labeled PMNs across a Transwell in response to 108 CFU/ml live PAO1, 108 CFU/ml HKPAO1 or 10 nM fMLP was not affected by the presence of 20 μM calpeptin. Data is presented as number of PMNs multiplied by 104 and represents mean ± s.d. of sextuplicate samples of one representative from three independent experiments. (P< 0.05, P<0.001; Student’s t-test).