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. Author manuscript; available in PMC: 2009 Dec 26.
Published in final edited form as: Cell. 2008 Dec 11;135(7):1276–1286. doi: 10.1016/j.cell.2008.11.028

Figure 5. Relaxation of pre-assembled dynamin scaffold by GTP.

Figure 5

A. Simultaneous addition of dynamin and GTP caused a slight gradual narrowing of wNT (red). At the new steady-state a decrease of wNT length (black) leads to the increase of its normalized conductance (Gn) indicating that wNT is not constrained by dynamin (see Figure 1C); B. Addition of GTP to “squeezed” nanotubes triggers an increase of both NT (black) and wNT (red) conductances. NT broke after a period of expansion, while wNT remained stable and was rapidly squeezed upon removal of GTP. C. Histogram showing steady-state conductance of NT (black) and wNT (red) upon addition of dynamin alone (dyn), GTP to nanotubes squeezed by dynamin (dyn, then GTP), dynamin and GTP (dyn+GTP) and dynamin and GDP (dyn+GDP); for dynamin and GTP the NT conductance was averaged near the fission point, error bars show SE. D. Effect of dynamin on the NT conductance is nucleotide-dependent: GTP stimulates fast fission of NT (black); GDP impairs both fission and curvature activities of dynamin.