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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Struct Biol. 2009 May;166(2):205–213. doi: 10.1016/j.jsb.2009.01.004

Figure 2.

Figure 2

Example of the DoG picker technique for picking particles of different sizes. The original image of GroEL particles (upper right) is first blurred by a sigma of 29.2Å (upper left). Next the image is successively blurred three more times by the values at the far left to get final images blurred by the amount below the image (left column). Each successive image pair is then subtracted to get the DoG filtered map (right column) highlighting the particles of a different size for each DoG map. This example then picks particles of size 37.5Å, 65.0Å, and 112.7Å.