The interactive TiltPicker display with pop-up dialogs. (A) The main window, both images are displayed side-by-side. The images are masked from the original square shape such that only the overlapping regions are shown. (B) Tilt angle calculator showing the calculated tilt angle, minimum triangle area and number of triangles used in the calculation. (C) The tilt parameters least-squares minimization refinement window. The six parameters are shown with their current values and the lock button (right column) to fix individual values during refinement. (D) The DoG Picker window with four options: particle size, threshold, particle contrast, and maximum number of peaks.