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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Arch Biochem Biophys. 2009 May 1;485(1):16–23. doi: 10.1016/j.abb.2009.01.014

Figure 5.

Figure 5

Detection of CPAF processing using a Western blot. The various GST-CPAF fusion proteins were loaded onto a SDS PAGE gel as described in the legend to Figure 3. After electrophoresis, the proteins bands were transferred onto nitrocellulose membrane for detection with the anti-CPAFc mAb 100a. All CPAF preps displayed the full-length GST-CPAF fusion protein along with fragments or varying length. However, only the Wt (lanes 2-4) and the unrelated CPAF mutant K540A (lane 17) displayed a protein band migrating at the position similar to band of CPAFc from the L2S100 sample (lanes 1 & 14).