Fig. 2.

An ACAT inhibitor reduces the induction of apoptosis by 7KC and oxidized low density lipoprotein (OxLDL) in isolated peritoneal macrophages. A: The effect of 58-035 on the induction of terminal transferase-mediated dUTP nick end labeling (TUNEL)-positive cells after a 24 h treatment with 7KC treatment. Resident peritoneal macrophages were isolated, plated on glass cover slips, and allowed to attach. The media was changed and supplemented with or without 58-035 (10 μg/ml) for 1 h before supplementation with and without 7KC (10 μg/ml), as indicated. The incubations were continued for 24 h. Apoptosis was then assayed by in situ TUNEL analysis as described in Materials and Methods. B: The effect of 58-035 on the induction of TUNEL-positive cells after 24 and 48 h incubations with OxLDL. Isolated resident peritoneal macrophages were supplemented with and without 58-035 (10 μg/ml) for 1 h before the addition of OxLDL (50 μg/ml). The incubation was continued for 24 and 48 h, as indicated. The induction of apoptosis was determined by in situ TUNEL analysis. The results are presented as mean percentages of TUNEL-positive cells ± SD in 10 random fields for each condition. The results shown are representative of three independent experiments. * P < 0.01 and ** P < 0.001 versus 7KC- or OxLDL-treated controls.