Fig. 5.

58-035, 5,8,11,14-eicosatetraynoic acid (ETYA), and arachidonyl trifluoromethyl ketone (AACOCF₃) inhibit the formation of 7KC esters in P388D1 cells. A: The effects of 58-035, ETYA, and AACOCF₃ on the accumulation of [³H]7KC esters in P388D1 cells. P338D1 cells were cultured in the presence of [³H]7KC (1.0 μCi/ml) in media supplemented with 58-035 (10 μg/ml), ETYA (20 μM), or AACOCF₃ (20 μM), as indicated. Unlabeled 7KC (10 μg/ml) was added and the cells were incubated for 16 h. Total lipids were extracted, and the radioactivity incorporated into 7KC esters was determined. B: The effects of a cyclooxygenase-1 inhibitor, FR-122047 (10 μM), and a cyclooxygenase-2 inhibitor, NS-398 (5 μM), on the accumulation of [³H]7KC esters in P388D1 cells were compared with treatment with 58-035 and ETYA. P388D1 cells were cultured in the presence or absence of the indicated inhibitors for 1 h before supplementation with [³H]7KC or unlabeled 7KC for 16 h, as described for A. * P < 0.05, ** P = 0.12, and *** P = 0.33 versus oxysterol-treated controls. Error bars represent standard deviation.