Selected reaction monitoring chromatographic traces of a standard mixture (A) containing 16:0, 18:0, 18:1, 18:2, and 20:4 7KC esters, as described in Materials and Methods. Selected reaction monitoring traces of a macrophage total lipid extract (B) represent analysis of ∼1.4% of the total extract. The top panel in each set contains overlaid selected reaction monitoring traces for 16:0 and 16:1; the middle panel contains traces for 18:0, 18:1, 18:2, and 18:3; and the bottom panel contains traces for 20:0, 20:1, and 20:4. The amplification factors for each trace relative to 7KC-18:0 are shown in parentheses; the same factor was applied to each selected reaction monitoring trace in each panel.