TABLE 1.
7KC ester content of P388D1 cells
| 7KC Estera | Normalized Amount | Absolute Mass |
|---|---|---|
| % | pmol/mg cell protein | |
| 7-Ketocholesteryl palmitate (16:0) | 20.98 ± 0.36 | 66.42 ± 7.15 |
| 7-Ketocholesteryl stearate (18:0) | 7.26 ± 0.88 | 22.81 ± 1.18 |
| 7-Ketocholesteryl oleate (18:1) | 71.12 ± 1.16 | 225.26 ± 25.57 |
| 7-Ketocholesteryl linoleate (18:2) | 0.22 ± 0.13 | 0.71 ± 0.51 |
| 7-Ketocholesteryl arachidonate (20:4) | 0.43 ± 0.13 | 1.35 ± 0.45 |
7KC ester, 7-ketocholesteryl ester. The MS response factors were calculated as described in Materials and Methods. P388D1 cells were incubated for 16 h in media supplemented with 20 μ/ml 7-ketocholesterol (7KC). The cells were rinsed twice with PBS and total lipids were extracted for mass spectral analysis as described in Materials and Methods. Data provided are of individual 7KC esters expressed as percentages of the total measured oxysteryl ester pool in the cell and as the absolute masses of the detected oxysteryl ester. All data are means ± SD of triplicate samples.
a
The numbers in parentheses refer to the length of the carbon chain and the number of double bonds of the fatty acyl chain.