Table 1. The primer sets for RT-PCR.
| Gene | Primer sequences | Annealing temp. (°C) | Product size (bp) |
|---|---|---|---|
| VCAM-1 | F: 5-CATCCACAAAGCTGCAAGAA-3 | 58 | 529 |
| R: 5-GCCACCACTCATCTCGATTT-3 | |||
| MMP2 | F: 5-GCGACAAGAAGTATGGCTTC-3 | 58 | 390 |
| R: 5-TGCCAAGGTCAATGTCAGGA-3 | |||
| MT1-MMP2 | F: 5-GGCAACATAATGAAATCACTT-3 | 58 | 301 |
| R: 5-TCGGCAGAGTCAAAGTGGGT-3 | |||
| MMP7 | F: 5-AAACTCCCGCGTCATAGAAAT-3 | 58 | 395 |
| R: 5-TCCCTAGACTGCTACCATCCG-3 | |||
| β-Actin | F: 5-GCACTCTTCCAGCCTTCCTTC-3 | 58 | 237 |
| R: 5-GGAGTACTTGCGCTCAGGAGG-3 |
Abbreviations: MMP, matrix metalloproteinase; MT1-MMP2, membrane type-1-MMP-2; RT-PCR, reverse transcription-polymerase chain reaction; temp., temperature; VCAM, vascular-cell adhesion molecule.